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赭曲霉毒素A对体外培养人胃黏膜上皮细胞染色体的损伤作用

崔晋峰, 吴 莎, 刘 静, 王 媛, 张祥宏

崔晋峰, 吴 莎, 刘 静, 王 媛, 张祥宏. 赭曲霉毒素A对体外培养人胃黏膜上皮细胞染色体的损伤作用[J]. 肿瘤防治研究, 2014, 41(06): 541-544. DOI: 10.3971/j.issn.1000-8578.2014.06.007
引用本文: 崔晋峰, 吴 莎, 刘 静, 王 媛, 张祥宏. 赭曲霉毒素A对体外培养人胃黏膜上皮细胞染色体的损伤作用[J]. 肿瘤防治研究, 2014, 41(06): 541-544. DOI: 10.3971/j.issn.1000-8578.2014.06.007
CUI Jinfeng, WU Sha, LIU Jing, WANG Yuan, ZHANG Xianghong. Damage Effects of Ochratoxin A on Chromosome of Human Gastric Epithelium Cells in vitro[J]. Cancer Research on Prevention and Treatment, 2014, 41(06): 541-544. DOI: 10.3971/j.issn.1000-8578.2014.06.007
Citation: CUI Jinfeng, WU Sha, LIU Jing, WANG Yuan, ZHANG Xianghong. Damage Effects of Ochratoxin A on Chromosome of Human Gastric Epithelium Cells in vitro[J]. Cancer Research on Prevention and Treatment, 2014, 41(06): 541-544. DOI: 10.3971/j.issn.1000-8578.2014.06.007

赭曲霉毒素A对体外培养人胃黏膜上皮细胞染色体的损伤作用

基金项目: 国家自然科学基金资助面上项目(81171889);国家自然科学基金青年科学基金资助项目(81301695);河北省自然科学基金青年科学基金资助项目(H2012206053)
详细信息
    作者简介:

    崔晋峰(1980-),女,博士,主治医师,主要从事肿瘤病因与肿瘤病理方面的研究

    通讯作者:

    张祥宏,E-mail:zhangxianghong2008@163.com

  • 中图分类号: R735.2

Damage Effects of Ochratoxin A on Chromosome of Human Gastric Epithelium Cells in vitro

  • 摘要: 目的 探讨赭曲霉毒素A(OTA)对人胃黏膜上皮细胞(GES-1)染色体的损伤作用。方法 采用微核试验观察不同浓度OTA(5、10、20 μmol/L)处理24 h时对GES-1细胞染色体的损伤情况。利用染色体核型分析进一步观察不同剂量OTA作用后GES-1细胞染色体的畸变情况。结果 微核试验结果显示10和20 μmol/L OTA处理后,GES-1细胞微核率分别为(2.90±0.54)%和(3.84±1.06)%,明显高于溶剂对照组[(1.23±0.27)%, P<0.05]。染色体核型分析表明OTA处理可以明显增加GES-1细胞染色体的畸变率,其中5、10、20 μmol/L OTA处理后GES-1细胞染色体总畸变率分别为14%、20%和24%,明显高于溶剂对照组4%。 结论 OTA处理可以增加GES-1细胞微核的形成,诱导GES-1细胞染色体发生畸变。

     

    Abstract: Abstract: Objective To explore the damage effect of ochratoxin A (OTA) on chromosome of human gastric epithelium cells (GES-1) in vitro. Methods The situation of chromosome damage was evaluated by micronucleus test after different concentrations of OTA treatments(5, 10, 20 μmol/L) for 24 h. The type of chromosome aberration was further observed by chromosome karyotypic analysis after OTA treatment at different concentrations. Results The micronucleus test results showed that the micronucleus rates in 10 and 20 μmol/L OTA treatment groups were (2.90±0.54)% and (3.84±1.06)% respectively, which were signifi cantly higher than that in control group [(1.23±0.27)%, P<0.05].Chromosome karyotypic analysis showed that total frequencies of aberrations were significant increased after OTA treatment. The total frequencies of aberrations were 14%, 20% and 24%, respectively in 5, 10 and 20 μmol/L OTA treated groups, which were all signifi cantly higher than that in control group (4%, P<0.05). Conclusion OTA could increase micronucleus rate and induce chromosome aberration in GES-1 cells.

     

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出版历程
  • 收稿日期:  2013-04-28
  • 修回日期:  2013-08-04
  • 刊出日期:  2014-06-24

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