甲状腺癌中CCR7和D2-40的表达及其与淋巴结转移的关系

杨润祥; 刘 林; 马 飞; 钱海利; 杨 芳

doi:10.3971/j.issn.1000-8578.2014.05.016

甲状腺癌中CCR7和D2-40的表达及其与淋巴结转移的关系

1.650118 昆明，昆明医科大学第三附属医院内二科；2.云南省第二人民医院；3.中国医学科学院肿瘤医院

基金项目: 国家自然科学基金资助项目（81360393）；云南省科技厅-昆明医科大学联合专项应用基础研究基金资助项目（2011FB203）

详细信息

作者简介:
杨润祥（1969-），女，硕士，教授，主要从事肿瘤内科疾病的基础与临床工作

中图分类号: R736.1
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出版历程
- 收稿日期: 2013-12-16
- 修回日期: 2014-02-19
- 刊出日期: 2014-05-24

Correlation between Expression of CCR7, D2-40 and Lymphatic Metastases in Thyroid Carcinoma

1.Chemotherapy Research Center, Yunnan Provincial Tumor Hospital, Kunming Medical University, Kunming 650118, China; 2.The Second People's Hospital of Yunnan Province;3.Cancer Hospital,Chinese Academy of Medical Sciences

摘要

摘要: 目的研究趋化因子受体7（CCR7）及D2-40在甲状腺癌组织中的表达及其与淋巴结转移的关系。方法应用MAX Vision免疫组织化学法研究二者在甲状腺癌和正常甲状腺组织中的表达情况，并以淋巴管内皮标记物D2-40计算微淋巴管密度 (lymphatic microvessel density, MLVD)。结果 CCR7在87例甲状腺癌和正常甲状腺组织中阳性率分别为66.7%和15%；乳头状癌、髓样癌、滤泡癌、未分化癌CCR7阳性率分别为75.0%、70.0%、73.0%、25.0%，淋巴结阳性组（40⁄49）与淋巴结阴性组（18⁄38）CCR7阳性率分别为81.6%、47.4%（P＜0.05）。D2-40标记的MLVD计数在甲状腺癌和正常甲状腺组织中分别为5.72±2.89??2.60±1.14，甲状腺乳头状癌、髓样癌、滤泡癌、未分化癌中分别为7.55±2.29、6.60±1.75、4.27±1.10、1.00±0.34。甲状腺癌中淋巴结阳性组与淋巴结阴性组D2-40标记的MLVD计数分别为9.39±4.24、3.24±1.67（P<0.05）。CCR7阳性组MLVD（9.37±2.25）高于阴性组（5.51±2.47），差异有统计学意义 (P<0.05)。D2-40标记的MLVD与CCR7 表达呈正相关（r_s=0.645,P＜0.001）。CCR7、D2-40标记的MLVD 与淋巴结转移呈正相关，甲状腺癌中淋巴结转移阳性组CCR7、D2-40标记的MLVD 高于淋巴结转移阴性组。结论肿瘤细胞高表达CCR7，D2-40能标记肿瘤淋巴管，联合监测CCR7与D2-40有望成为判断淋巴转移更为有效的指标。
- 甲状腺癌 /
- 趋化因子受体7 /
- D2-40 /
- 微淋巴管密度 /
- 淋巴转移
Abstract: Objective To investigate the relationship between expression of CCR7, D2-40 and lymph node metastasis in thyroid carcinoma tissues. Methods The expression of CCR7 and D2-40 were measured in 87 cases of thyroid carcinoma tissues and 20 cases of normal thyroid tissues by MAX Vision immunohistochemical staining technique. The expression of lymphatic endothelial markers D2-40 was applied to calculate lymphatic microvessel density(MLVD). Results CCR7 positive rate was 66.7% in the 87 cases of thyroid carcinoma tissues and CCR7 positive rate was 15% in the 20 cases of normal thyroid tissues; CCR7 positive rates were counted up to 75.0%, 70.0%, 73.0% and 25.0% in PTC, MTC, FTC and UTC, retrospectively; CCR7 positive rate was 81.6%（40⁄49）in lymph node metastasis positive patients and 47.4% (18⁄38) in lymph node metastasis negative patients(P<0.05). MLVD counts in thyroid carcinoma tissues and normal tissues were (5.72±2.89) and (2.60±1.14); MLVD counts in PTC, MTC, FTC and UTC were (7.55±2.29), (6.60±1.75), (4.27±1.10) and (1.00±0.34), respectively; MLVD counts in lymph node metastasis positive patients was obviously higher than that in lymph node metastasis negative patients [(9.39±4.24), (3.24±1.67), P<0.05]. MLVD expression was positively correlated with CCR7 expression ( r_s=0.645，P<0.001). CCR7 and MLVD were positively related with lymphatic metastasis. The positive rates of CCR7 and MLVD in lymph node-positive group were higher than those in lymph node-negative group. Conclusion D2-40 staining only presents in lymphatic endothelial cells. The united detection of CCR7 along with D2-40 is expected to be the inchoate and more effective signal to judgment lymph node metastases.
- Thyroid carcinoma /
- CCR7 /
- D2-40 /
- MLVD /
- Lymphatic metastases

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参考文献(26)

[1]	Ren LJ,Yang YF,Zhang CL. Clinical research on regularity of lymph node metastasis in papillary thyroid carcinoma[J]． Zhongguo Pu Wai Ji Chu Yu Lin Chuang Za Zhi, 18（4）:419 -22.[ 任立军,杨延芳,张成雷.甲状腺乳头状癌淋巴结转移规律的临床研究[J].中国普外基础与临床杂志,2011,18(4):419-22.]
[2]	Müller A, Homey B, Soto H, et al. Involvement of chemokine receptors in breast cancer metastasis[J]. Nature,2002,410(6842): 50-6.
[3]	Mashino K, Sadanaga N, Yamaguchi H, et al. Expression of chemokine receptor CCR7 is associated with lymph n o d e m e t a s t a s i s o f g a s t r i c c a r c i n o m a [ J ] . C a n c e r Res,2002,62(10):2937-41.
[4]	Yang RX, Ma F, Li M,et al.Expression and its value of chemokine recepter CCR7 expression in breast cancer[J] . Lin Chuang Zhong Liu Xue Za Zhi, 2007,12(12):906-8. [杨润祥,马飞,李梅,等.乳腺癌组织中趋化因子受体CCR7的表达及其意义[J]. 临床肿瘤学杂志, 2007,12(12):906-8.]
[5]	Cabioglu N, Yazici MS, Arun B, et al. CCR7 and CXCR4 as novel biomarkers predicting axillary lymph node metastasis in T1 breast cancer[J]. Clin Cancer Res,2005,11(16):5686-93.
[6]	Andre F, Cabioglu N, Assi H, et al. Expression of chemokine receptors predicts the site of metastatic relapse in patients with axillary node positive primary breast cancer[J]. Ann Oncol,2006,17(6):945-51.
[7]	Weninger W,Carlsen HS,Goodarzi M,et a1.Naive T cell recruitment to non-lymphoid tissues：a role for endothelium expressed CC ahemokine ligand 21 in autoimmune disease and lymphoid neogenesis[J].J Immunol,2003,170(9):4638-48.
[8]	Kahn HJ, Marks A. A new monoclonal antibody, D2-40, for detection of lymphatic invasion in primary tumors[J]. Lab Invest, 20 02,82(9):1255-7.
[9]	Feng WN, Gu LJ, Deng YM, et al. The prognostic value of lymphatic vessel invasion makerd by D2-40 in non-small lung cancer[J]. Shi Yong Yi Xue Za Zhi, 2009(25)14:2295-7.[ 冯卫能, 谷力加,邓燕明,等.D2-40标记的淋巴管侵犯在非小细胞肺癌中的预后价值[J]. 实用医学杂志,2009,(25)14:2295-7.]
[10]	Chen P, Zhang YH, Han Y, et al. D2-40 expression and clinical signifi cance in breast cancer[J]. Lin Chuang Yu Shi Yan Bing Li Xue Za Zhi, 2010，26(1):101-2.[ 陈萍,张玉华,韩影,等.D2-40 在乳腺癌中的表达及临床意义[ J ] .临床与实验病理学杂志,2010,26(1):101-2.]
[11]	Li Y, Zhang DY. Expression and clinical significance of D2-40 in colorectal adenocarcinoma[J].Shanxi Yi Ke Da Xue Xue Bao, 20 09,40(8):696-9. [李英,张冬娅.D2-40在结直肠腺癌中的表达及临床意义[J].山西医科大学学报,2009,40(8):696-9.]
[12]	Guo YL, Sun PP, Guo W, et al. The expression and clinical signifi cance of VEGF-C,VEGF-D and D2-40 in gastric cardiac adenocarcinoma[J].Lin Chuang Yu Shi Yan Bing Li Xue Za Zhi,2009,25(1):81-3. [郭艳丽,孙萍萍,郭炜,等.贲门腺癌中 VEGF-C、VEGF-D和D2-4的表达及意义[J].临床与实验病学杂志,2009,25(1):81-3.]
[13]	Feng ZH, Li CQ, Yang L,et al. Expression of D2-40 in oral squamous carcinoma and its clinical signifi cance[J].Zhongguo Zu Zhi Hua Xue Yu Xi Bao Hua Xue Za Zhi, 2009,18(6):701-4. [冯正虎,李春青,杨兰,等.口腔鳞癌中D2-40表达的特点及临床意义 [J] 中国组织化学与细胞化学杂志,2009,18(6):701-4.]
[14]	Shields JD, Fleury ME, Yong C,et al. Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial fl ow and autocrine CCR7 signaling[J]. Cancer Cell,2007,11(6):526-38.
[15]	Wang SQ, Zhang F, Li P, et al. Relationship between expression of chemokine CCR7, CCL21 and lymphatic metastasis in cervical cancer[J]. Zhongguo Fu You Bao Jian, 2010,25(21):3019-22. [王淑强,张凡,李鹏,等.子宫颈癌中趋化因子 CCR7、CCL21 表达与淋巴转移的关系[J].中国妇幼保健,2010,25(21):3019-22.]
[16]	Zhao ZQ, Yu ZY, Xie QC. Expression of VEGF-C in the serum of NSLC patients and its clinicopathologic signifi cance[J]. Zhongguo Zhong Liu Lin Chuang, 2008,35(12):690-2. [赵忠全,余宗阳,谢启超.非小细胞肺癌患者血清血管内皮生长因子C的水平及临床意义[J] 中国肿瘤临床,2008,35(12):690-2.]
[17]	Yang HP, Wu ES, Chen QL, et al. Expression of VEGF-C in non-small cell lung cancer[J].Yi Xue Lin Chuang Yan Jiu, 2008,25(8):1423-5.[杨华平,吴鄂生,陈清兰,等.非小细胞肺癌血管内皮生长因子C表达的研究[ J ] . 医学临床研究,2008,25(8):1423-5.]
[18]	Dong X, Qiu XS ,Wang EH,et al. Expression of vascular endothelial growth factor(VEGF) C and VEGF receptor 3 in non-small cell lung cancer[J]. Zhonghua Bing Li Xue Za Zhi, 20 03,32(2):128-32. [董昕,邱雪杉,王恩华,等.血管内皮生长因子 C及其受体3在非小细胞肺癌组织中的表达及意义[J].中华病理学杂志,2003,32(2):128-32.]
[19]	Zhang WD, Xiao QM, Hu CP, et al. The Expression and Clinical Significance of Vascular Endothelial Growth Factor-C in Non-small Cell Lung Cancer[J]. Zhongguo Yi Shi Za Zhi, 20 04,6(3):342-4. [张卫东,肖奇明,胡成平,等.血管内皮生长因子C在非小细胞肺癌中的表达[ J ] . 中国医师杂志， 20 04,6(3):342-4.]
[20]	Huang H, Zhang CQ, Zhao L. The expression of chemokine receptor CCR7 in colorectal cancer and its clinical signifi cance[J]. Zhejiang Lin Chuang Yi Xue, 2010,12(2):116-8.[黄河,张才全,赵林.趋化因子受体CCR7在结直肠癌中的表达及临床意义[J].浙江临床医学,2010,12(2):116-8.]
[21]	Zhao B, Zhao WH, Zhang B, et al. Quantitative analys and significance of secondary lymphoid-tissue chemokine, CCR7 and VEGF-C expression in pancreatic cancer and their clinical significance[J]. Zhonghua Zhong Liu Fang Zhi Za Zhi, 20 08,15(17):1289-93.[赵斌,赵文华,张波,等.胰腺癌组织 S L C 及其受体 CCR7与VEGF-C表达及相关性研究[J]. 中华肿瘤防治杂志,2008,15(17):1289-93.]
[22]	Onguru O,Kurt B,Gunhan O,et al.Cyclooxygenase-2(COX-2) expression and angiogenesis in intraeranial ependymomas[J].Clin Neurpathol, 2006,25(5):216-20.
[23]	Simeone AM,Nieves-Alicea R,McMurtry VC, e t a l . Cyelooxygenase-2 uses the protein kinaseC/interleukin-8/ urokinase-type plasminogen activator pathway to increase the invasiveness of breast cancer cells[J]. Int J Oneol,2007,30(4):785-92.
[24]	Siironen P, Ristimäki A,Narko K,et al. VEGF-C and COX-2 expression in papillary thyroid caneer[J]. Endocr Relat Cancer, 20 06,13(2):465-73.
[25]	Wang WJ, Bai JL, Ji P. The signifi cance of lymphangiogenesis in lymph node metastasis of bladder transitional cell carcinoma[J]. Shi Yong Yi Xue Za Zhi, 2007, 23 (16):2494-6. [王文进,白进良, 纪鹏.淋巴管新生在膀胱移行细胞癌淋巴结转移中的意义[J]. 实用医学杂志,2007, 23 (16):2494-6.]
[26]	Yang RX, Ren HX,Duan LC, et al.Relationship between D2-40 and CCR7 expression and lymph node metastasis in non-small cell lung cancer[J]. Zhong Liu Fang Zhi Yan Jiu,2011,38(8):921-5. [杨润祥,任宏轩,段林灿,等.非小细胞肺癌中D2-40、CCR7的表达与淋巴结转移的关系[J]. 肿瘤防治研究, 2011,38(8):921-5.]

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甲状腺癌中CCR7和D2-40的表达及其与淋巴结转移的关系

作者简介:
杨润祥（1969-），女，硕士，教授，主要从事肿瘤内科疾病的基础与临床工作

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Correlation between Expression of CCR7, D2-40 and Lymphatic Metastases in Thyroid Carcinoma

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甲状腺癌中CCR7和D2-40的表达及其与淋巴结转移的关系

作者简介: 杨润祥（1969-），女，硕士，教授，主要从事肿瘤内科疾病的基础与临床工作

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Correlation between Expression of CCR7, D2-40 and Lymphatic Metastases in Thyroid Carcinoma

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作者简介:
杨润祥（1969-），女，硕士，教授，主要从事肿瘤内科疾病的基础与临床工作