Abstract: Objective To explore the relationship between aberrantly expressed genes in lung cancer genes and after chemotherapy by gene expression profi le. Methods In this article, expression profi le of lung cancer tissue and normal lung tissue were compared to screen out the differentially expressed genes by statistical method. The aberrant expressed genes of lung cancer cells were clarifi ed. Then the up-regulated aberrantly expressed genes were upregulated by the chemotherapy drugs were selected by association analysis. Finally, the genes which could correspond to many chemotherapy drugs were screened and analyzed by network regulation, to clarify the possible regulation relationship between these genes. Results Three hundred and ninety-seven up-regulated aberrantly expressed genes induced by 6 kinds of related chemotherapy drugs in lung cancer tissue were screened out. Among them, TOP2A，MAD2L1，BIRC5 corresponded to many chemotherapy drugs and MAD2L1 may control the biological function of TOP2A and BIRC5 through P53. Conclusion The up-regulated expression genes, TOP2A，MAD2L1 and BIRC5, may have correlation with the effect of chemotherapy of lung cancer. MAD2L1 may infl uence the biological function of TOP2A and BIRC5.