Abstract: Obiective To investigate the impaction of C-terminal binding protein 1 (CtBP1) on proliferation of hepatocellular carcinoma cell HepG2 and related mechanism. Methods siRNA targeting for CtBP1 were introduced into HepG2 cells to knock down CtBP1,3 control groups including blank control, transfection reagent and control siRNA were used as controls.At different time points after transfection, HepG2 cells were collected for MMT assay to detect cell proliferation and fl owcytometry to detect cell cycle distribution and apoptosis. Results CtBP1 siRNA effectively knocked down CtBP1 in HepG2 cell. Compared with control group, CtBP1 protein signifi cantly was decreased by 57.80%.Cell growth were signifi cantly inhibited after CtBP1 knockdown (P＜0.05). The inhibition rates of cell growth were 22.34%,52.15%,53.97% and 54.77% at 24, 48, 72 and 96 h respectively. The distribution of cell cycle was not changed but apopotic cells were increased significantly compared with control group. Conclusion CtBP1 knockdown could inhibit cell proliferation of HepG2 mainly through apoptosis instead of cell cycle arrest. CtBP1 is involved in cell growth and apoptosis inhibition of tumor cells, and could be a candidate for cancer targeted therapy.