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人巨细胞病毒感染对围手术期尿路上皮癌患者免疫功能的影响

李法锦, 施浩强, 唐 亮, 张 涛, 樊 松, 江长琴, 葛 俊, 王明丽, 梁朝朝

李法锦, 施浩强, 唐 亮, 张 涛, 樊 松, 江长琴, 葛 俊, 王明丽, 梁朝朝. 人巨细胞病毒感染对围手术期尿路上皮癌患者免疫功能的影响[J]. 肿瘤防治研究, 2014, 41(02): 119-123. DOI: 10.3971/j.issn.1000-8578.2014.02.006
引用本文: 李法锦, 施浩强, 唐 亮, 张 涛, 樊 松, 江长琴, 葛 俊, 王明丽, 梁朝朝. 人巨细胞病毒感染对围手术期尿路上皮癌患者免疫功能的影响[J]. 肿瘤防治研究, 2014, 41(02): 119-123. DOI: 10.3971/j.issn.1000-8578.2014.02.006
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LI Fajin, SHI Haoqiang△, TANG Liang, ZHANG Tao, FAN Song, JIANG Changqin, GE Jun, WANG Mingli, LIANG Chaozhao. Impact of Human Cytomegalovirus Infection on Immune Function of Urothelial Carcinoma Patients in Perioperative Period[J]. Cancer Research on Prevention and Treatment, 2014, 41(02): 119-123. DOI: 10.3971/j.issn.1000-8578.2014.02.006
Citation: LI Fajin, SHI Haoqiang△, TANG Liang, ZHANG Tao, FAN Song, JIANG Changqin, GE Jun, WANG Mingli, LIANG Chaozhao. Impact of Human Cytomegalovirus Infection on Immune Function of Urothelial Carcinoma Patients in Perioperative Period[J]. Cancer Research on Prevention and Treatment, 2014, 41(02): 119-123. DOI: 10.3971/j.issn.1000-8578.2014.02.006
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人巨细胞病毒感染对围手术期尿路上皮癌患者免疫功能的影响

  • 1.230022合肥，安徽医科大学微生物教研室；2.安徽医科大学第一附属医院泌尿外科；3.安徽医科大学第二附属医院泌尿外科（*：现单位：550004 贵阳，贵州省疾病预防控制中心传染病防治研究所检验科）

基金项目: 国家临床重点专科（泌尿外科）建设项目
详细信息
    作者简介:

    李法锦（1986-），女，硕士，主管技师，主要从事临床分子病毒的研究

    施浩强（1971-），男，硕士，副教授，副主任医师，主要从事膀胱癌研究（△：并列第一作者）

    通讯作者:

    梁朝朝，E-mail：liang_chaochao@163.com

  • 中图分类号: R 737.14

Impact of Human Cytomegalovirus Infection on Immune Function of Urothelial Carcinoma Patients in Perioperative Period

  • 1.Department of Microbiology, Anhui Medical University, Hefei 230032, China; 2.Department of Urology, The First Affiliated Hospital of Anhui Medical University; 3.Department of Urology, The Second Affi liated Hospital of Anhui Medical University(*: Present: Laboratory of National Institute for Communicable Disease Control and Prevention, Guizhou Center for Disease Control and Prevention, Guiyang 550004, China)

摘要

    摘要
  • 摘要: 目的 检测围手术期尿路上皮癌患者外周血的CD3+、CD4+、CD8+T淋巴细胞比例和人类巨细胞病毒（HCMV）IgG和IgM抗体，了解近期HCMV感染对围手术期尿路上皮癌患者细胞免疫功能的影响。方法 采集33例经病理检测确诊为尿路上皮癌的患者外周血，流式细胞仪检测外周血CD3+、CD4+和CD8+ T细胞亚群比例，血浆ELISA法定性检测HCMV-IgG和HCMV-IgM抗体，根据HCMV-IgM抗体结果将尿路上皮癌患者分为阳性组和阴性组，比较两组患者的T细胞亚群有无差异。结果 33例患者中HCMV-IgG抗体阳性31例；HCMV-IgM抗体阳性7例，阴性26例。HCMV-IgM阳性组和阴性组之间的CD3+、CD4+ 和CD8+ T细胞亚群有着显著的差异（P<0.01）。阳性组患者的CD4+ T细胞的百分比显著低于阴性组，并且该组CD4+/CD8+比值亦显著低于阴性组。CD4+/CD8+的比值提示阳性组中有85%（6/7）的患者处于免疫功能低下状态，而相对于阴性组仅有11.5%（3/23）的患者处于免疫功能低下状态，两组间差异有统计学意义（P<0.01）。结论 术前检测HCMV-IgM可以很好地预测尿路上皮癌患者的细胞免疫功能，对术后辅助治疗具有指导意义。

     

    Abstract: Objective To detect the ratio of CD3+, CD4+, CD8+ T lymphocytes and HCMV-IgG and IgM antibodies in peripheral blood of urothelial carcinoma patients in perioperative period and to evaluate the impact of HCMV infection on immune functions. Methods Thirty-three peripheral blood samples were collected from urothelial carcinoma patients with pathological diagnosis. Flow cytometry was performed to detect the ratio of CD3+, CD4+ and CD8+ T lymphocytes subsets. ELISA was conducted to detect HCMVIgG and IgM antibodies. Patients were divided into positive group and negative group based on HCMVIgM results. T lymphocyte difference was also compared. Results HCMV-IgG antibodies of 31 patients were positively expressed, HCMV-IgM antibodies of 7 patients were positively expressed, and HCMV-IgM antibodies of 26 patients were negatively expressed respectively. CD3+, CD4+ and CD8+ T lymphocytes subsets were statistically different between HCMV-IgM positive and negative groups(P<0.01). The percentage of CD4+ T lymphocytes and CD4+/CD8+ ratio of positive group were obviously less than those of negative group. CD4+/CD8+ ratio showed that the immune function of 85% (6/7) positive group was poor, while there was only 11.5% (3/23) in negative group. There was a statistical difference between two groups (P<0.01). Conclusion Detecting HCMV-IgM preoperatively may predict the cellular immune functions of patients with urothelial carcinoma, contributing to perform adjuvant therapy after operation

     

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出版历程
  • 收稿日期:  2013-09-02
  • 修回日期:  2013-10-29
  • 刊出日期:  2014-02-24

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