Abstract: Objective To observe the effects of GNP (goldnano particles,GNP) on nude mice hepatic tumor vascular morphology and the expression of hypoxia inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) mRNA. Methods Hepatocellular cancer cell line H22 were injected subcutaneously into the right armpits of 14 Balb/c nude mice. When the diameter of transplanted tumor reached about 7-8 mm,they were randomly divided into 2 groups, and drugs with GNP or normal saline were injected respectively. For 7 days later, the tumor vascular diameter and blood volume were measured by Doppler ultrasound. When the mice were sacrificed, the tumor's size was calculated and the expression of HIF-1α and VEGF mRNA were detected by in situ hybridization. Results (1)The tumor's size in experiment group (0.935±0.129 cm³) was decreased in comparison with that in control group (1.573±0.247 cm3), there was significantly difference (P<0.05).(2) The tumor's vascular diameter and blood volume in experiment group were (0.6397±0.1548)mm and (1.171±0.241)cm³ respectively, they were all signifi cantly reduced in comparison with those in control group (1.1000±0.3247 mm, 2.357±0.408 cm3)(P<0.05). (3)The expression of HIF-1α mRNA (15.3±7.4%) and VEGF mRNA (23.7±9.5), were dramatically attenuated in comparison with those in control group (67.2±13.1)%,(70.3±14.6)% (P<0.05). Conclusion GNP can make nude mice tumor's vascular morphology better and decrease their blood volume, inhibit the expression of HIF-1α mRNA,VEGF mRNA, this situation is benefi t to restrain the tumor growth.