Abstract: Objective To investigate the potential mechanisms of Feiyanning combined with Gefitinib inhibiting growth of lung adenocarcinoma cell H1975. Methods H1975 human lung adenocarcinoma cells were implanted subcutaneously in the left armpits of male athymic nude mice to establish transplantation tumor models of human lung cancer. Forty models were randomly and evenly divided into four groups, Control group, Feiyanning group (F), Gefi tinib (G) group and F+G group, and treated with corresponding drugs for 4 weeks. Tumor volume, tumor weight and body weight were measured to draw up tumor growth curves and calculated the tumor weight inhibition rates. The aoptotic rates were measured by TUNEL stain method, and the expressions of EGFR-PI3K/AKT pathway related proteins were detected by Western blot. Results The tumor weight inhibition rates were 0, 21.91%, 25.11%, 53.28% of groups of Control, Gefitinib, Feiyanning and F+G respectively. The apoptotic rate of F+G was（55.06±2.39）%, which was increased significantly compared with Control group（15.11±1.6）%, Gefitinib group （26.64±0.69）% and Feiyanning group（28.88±1.61）% (P<0.01). The result of Western blot showed that the expressions of p-EGFR, p-AKT and p-mTOR were decreased signifi cantly in F + G group. Conclusion Feiyanning combined with Gefi tinib may be blockade EGFR-PI3K/AKT pathway to inhibit the tumor growth and induce apoptosis of lung adenocarcinoma cell H1975 in xenograft nude mice models.