GATA-3 基因在白血病骨髓基质细胞中的表达
The Expression of Transcription Factor GATA-3 Gene in Bone Marrow Stromal Cells from Patients with Leukemia
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摘要: 目的 了解转录因子GATA-3 基因在白血病和正常骨髓基质细胞(BMSC) 中表达的情况。方法 体外扩增培养BMSC, 运用RT-PCR-EL ISA 方法检测GATA-3 基因的表达情况,并对其相对表达水平进行半定量比较。结果 GATA-3 基因在正常和白血病的BMSC 中均有一定的表达。BMSC 中急性髓性白血病(AML) 和慢性粒细胞白血病(CML) 组的GATA-3 基因表达率与正常组比较无明显差异,而急性淋巴细胞白血病(ALL) 组的GATA-3 表达率比正常组低,且AML BMSC 中GATA-3 基因表达水平> ALL > 正常> CML 。结论 转录因子GATA23 基因可在正常和白血病骨髓基质细胞中表达,并可能影响骨髓微环境的造血调控作用,其表达模式的改变可能与白血病发生发展有一定的关系。Abstract: Objective To investigate the expression of t ranscription factor GATA-3 gene in the bone marrow st romal cells (BMSCs) f rom patients with leukemia or normal controls. Methods The expression of GATA-3 gene was analyzed by using RT2PCR2EL ISA in the BMSCs from 34 normal cases and 42 cases with leukemia. The semi-quantitative expression levels of GATA-3 gene in the BMSCs from patients with leukemia were compared with normal cont rols. Results The expression of GATA-3 gene could be detected in the BMSCs from both normal cont rols and the cases with leukemia. The expression rates of GATA-3 gene in the BMSCs from acute myelocytic leukemia (AML ) and chronic myelocytic leukemia (CML) were similar to the normal controls, whereas the expression rate in the BMSCs f rom acute lymphocytic leukemia (ALL) was significant lower than the normal cont rols ( P < 0. 05) . The rank of expression level of GATA-3 gene in the BMSCs was“AML > ALL > normal > CML”. Conclusion The expression of GATA-3 gene could be identified in the BMSCs f rom normal cont rols and patient s with leukemia. It may influence the regulation of hematopoiesis in the BMSCs and the change of expression pattern may play a certain role in pathogenesis and development of leukemia.
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