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外周血中TYMS和ERCC1基因多态性对胃肠道肿瘤患者化疗疗效的评估

张凤岐, 沈燕, 王珊珊, 卫肖

张凤岐, 沈燕, 王珊珊, 卫肖. 外周血中TYMS和ERCC1基因多态性对胃肠道肿瘤患者化疗疗效的评估[J]. 肿瘤防治研究, 2014, 41(08): 906-911. DOI: 10.3971/j.issn.1000-8578.2014.08.011
引用本文: 张凤岐, 沈燕, 王珊珊, 卫肖. 外周血中TYMS和ERCC1基因多态性对胃肠道肿瘤患者化疗疗效的评估[J]. 肿瘤防治研究, 2014, 41(08): 906-911. DOI: 10.3971/j.issn.1000-8578.2014.08.011
ZHANG Fengqi, SHEN Yan, WANG Shanshan, WEI Xiao. Significance of TYMS and ERCC1 Gene Polymorphism in Peripheral Venous Blood in Evaluating the Chemotherapeutic Effect on Patients with Gastroenteric Tumor Patients[J]. Cancer Research on Prevention and Treatment, 2014, 41(08): 906-911. DOI: 10.3971/j.issn.1000-8578.2014.08.011
Citation: ZHANG Fengqi, SHEN Yan, WANG Shanshan, WEI Xiao. Significance of TYMS and ERCC1 Gene Polymorphism in Peripheral Venous Blood in Evaluating the Chemotherapeutic Effect on Patients with Gastroenteric Tumor Patients[J]. Cancer Research on Prevention and Treatment, 2014, 41(08): 906-911. DOI: 10.3971/j.issn.1000-8578.2014.08.011

外周血中TYMS和ERCC1基因多态性对胃肠道肿瘤患者化疗疗效的评估

详细信息
    作者简介:

    张凤岐(1988-),女,硕士在读,主要从事分子诊断与临床研究工作

    通讯作者:

    沈燕,E-mail:shenyan62687@163.com

  • 中图分类号: R735

Significance of TYMS and ERCC1 Gene Polymorphism in Peripheral Venous Blood in Evaluating the Chemotherapeutic Effect on Patients with Gastroenteric Tumor Patients

  • 摘要: 目的 探究外周血TYMS与ERCC1基因多态性在评估胃肠道肿瘤患者化疗疗效中的意义。方法 实验分组:(1)未化疗患者标本:外周血组和肿瘤组织组。(2)化疗患者标本: 有效组和无效组。PCR和PCR-RFLP检测外周血与肿瘤组织TYMS、ERCC1基因型。结果 未化疗患者外周血与肿瘤组织中,TYMS基因3R/3R、(2R/2R、2R/3R)基因型检出率均为72.09%、27.91%(P<0.01);ERCC1 基因C/C、(T/T、C/T)基因型检出率均为81.39%、18.61%(P<0.01)。化疗患者外周血TYMS基因3R/3R、(2R/2R、2R/3R)基因型检出率为65.79%和34.21%,ERCC1基因C/C、(T/T、C/T)检出率为63.16%和36.84%;TYMS基因型携带者化疗有效率分别为18%和57.69%,ERCC1基因型携带者化疗有效率为47.91%和3.57%。 TYMS、ERCC1各基因型与化疗有效率相关(P<0.01),各基因型间化疗有效率差异有统计学意义(P<0.01)。结论 外周血标本可替代肿瘤组织检测TYMS和ERCC1基因多态性,且外周血TYMS和 ERCC1基因多态性可用于评估5-Fu和铂类药物疗效。

     

    Abstract: Objective To investigate the significance of TYMS and ERCC1 gene polymorphism in peripheral venous blood in evaluating chemotherapeutic effect on outcomes of patients with gastroenteric tumor. Methods Experimental groups: (1)Specimens of patients with non-chemotherapy groups: peripheral blood group and tumor tissue group. (2)Specimens of patients with chemotherapy groups: effective chemotherapy group and ineffective group. The genotypes of TYMS and ERCC1 in peripheral blood and tumor tissue were detected by PCR and PCR-RFLP. Results In peripheral blood and tumor tissues of patients with nonchemotherapy, the detection rates of 3R/3R, (2R/2R, 2R/3R) genotypes of TYMS were 72.09% and 27.91% (P<0.01), those of C/C, (T/T, C/T) genotypes of ERCC1 were 81.39% and 18.61% (P<0.01). The detection rates of 3R/3R, (2R/2R, 2R/3R) genotypes of TYMS in patients with chemotherapy were 65.79% and 34.21%; those of C/C, (T/T, C/T) genotypes of ERCC1 were 63.16% and 36.84%. Chemotherapy response rates of TYMS genotype carriers were 18% and 57.69%; those of ERCC1 were 47.91% and 3.57%. Genotypes of TYMS and ERCC1 had correlation with chemotherapy response rate (P<0.01). Chemotherapy response rates between the genotypes were different(P<0.01). Conclusion Tumor tissues could be replaced by peripheral blood sample in detecting TYMS and ERCC1 polymorphism which could be used to evaluate the efficacy of 5-Fu and platinum drugs.

     

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出版历程
  • 刊出日期:  2014-08-24

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