Role of Wnt/PCP Signal Pathway in Colon Carcinogenesis in Experimental Rats
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摘要: 目的 探讨Wnt/PCP信号通路在大鼠结肠癌形成过程中的作用，为结肠癌靶向治疗寻找新的靶点。方法 MNU(N-甲基-N-亚硝基脲)灌肠法诱导大鼠形成结肠肿瘤。RT-PCR技术检测Wnt/PCP信号通路中重要因子Wnt7a、JNK、RhoA在结肠癌组织、结肠非典型增生组织和正常结肠组织中的表达情况。结果 60只SD大鼠取标本后经病理证实，有28只大鼠形成非典型增生，23只形成结肠癌。Wnt7a、JNK和RhoA mRNA在结肠非典型增生组织及结肠癌组织中的表达高于正常结肠组织，差异有统计学意义。结论 Wnt/PCP信号通路在结肠癌发生发展过程中可能起重要作用。Abstract: Objective To explore the role of Wnt/PCP signal pathways in colon carcinogenesis in experimental rats, for new targets in the treatment of colon cancer. Methods SD rats colon cancer model was established by MNU enema method. RT-PCR was applied to test the expression of Wnt7a, JNK and RhoA, which are important factors in Wnt/PCP signal pathway, in colon cancer tissue, colon atypical hyperplasia tissue and normal colon tissue. Results A total of 60 SD rats specimens were confirmed pathologically that atypical hyperplasia were formed in 28 rats and colon cancer were formed in 23 rats. RT-PCR showed that the expression of Wnt7a, JNK and RhoA mRNA in colon atypical hyperplasia tissue and colon cancer tissue were higher than that in normal colon tissue, with statistical difference. Conclusion Wnt/PCP signal pathway may play an important role in colon carcinogenesis.
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Key words:
- Colon cancer /
- Wnt/PCP /
- Signal pathway
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[1] Zhou J, Cheng P, You JI, et al. Notch and wingless signaling cooperate in regulation of dendritic cell differentiation[J]. Immunity,2009,30(6): 845-59. [2] Tang WJ, Wu ZY, Yu DH. Experimental model for colorectal cancer[J]. Zhonghua Shi Yan Wai Ke Za Zhi, 2000,17(4):380-1. [ ΰ , ־ , º . ֱ ʵ ģ [J]. л ʵ ־,2000,17(4):380-1.] [3] Schwarz-Romond T. Three decades of Wnt signalling[J]. EMBO J, 20 12, 31(12):2664. [4] Takemaru KI, Ohmitsu M, Li FQ. An oncogenic hub beta-catenin as a molecular target for cancer therapeutics[J]. Handb Exp Pharmacol, 2008, (186) 261-84. [5] Ding SZ, Goldberg JB, Hatakeyama M. Helicobacter pylori infection, oncogenic pathways and epigenetic mechanisms in gastric carcinogenesis[J]. Future Oncol, 2010,6(5):851-62. [6] Velasco J, Zarrabeitia MT, Prieto JR, et al. Wnt pathway genes in osteoporosis and osteoarthritis: differential expression and genetic association study[J]. Osteoporos Int,2010, 21(1): 109-18. [7] Clément G,Guilleret I,He B,et al. Epigenetic alteration of the Wnt inhibitory factor-1 promoter occurs early in the carcinogenesis of Barrett’s esophagus[J]. Cancer Sci,2008, 99(1):46-53.
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