Abstract: Objective To explore the role of Wnt/PCP signal pathways in colon carcinogenesis in experimental rats, for new targets in the treatment of colon cancer. Methods SD rats colon cancer model was established by MNU enema method. RT-PCR was applied to test the expression of Wnt7a, JNK and RhoA, which are important factors in Wnt/PCP signal pathway, in colon cancer tissue, colon atypical hyperplasia tissue and normal colon tissue. Results A total of 60 SD rats specimens were confirmed pathologically that atypical hyperplasia were formed in 28 rats and colon cancer were formed in 23 rats. RT-PCR showed that the expression of Wnt7a, JNK and RhoA mRNA in colon atypical hyperplasia tissue and colon cancer tissue were higher than that in normal colon tissue, with statistical difference. Conclusion Wnt/PCP signal pathway may play an important role in colon carcinogenesis.