Expression of FMNL-2 and MMP-2 in Primary Hepatocellular Carcinoma and Its Clinicopathological Characteristics
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摘要: 目的 探讨肝癌及癌旁组织中FMNL-2和MMP-2的蛋白表达及与临床病理特征的关系。方法 采用免疫组织化学MaxvisionTM法检测100例肝癌及癌旁组织中FMNL-2和MMP-2的蛋白表达情况，应用图文分析软件Image-Pro Plus6.0测定FMNL-2和MMP-2的蛋白累积吸光度值（IOD），采用SPSS19.0 软件统计分析。结果 肝癌及癌旁组织中FMNL-2蛋白IOD值分别为(90 748.36±46 276.91)和(39 075.46±24 763.12)，MMP-2蛋白IOD值分别为（87 969.46±27 131.77）和（59 517.72±20 218.23），两组差异均有统计学意义（P<0.05）；FMNL-2在伴与不伴肝硬化组中IOD值分别为（47 471.96±40 273.54）和（72 123.41±45 517.93）（P<0.05）；MMP-2在HBsAg阳性或阴性组中IOD值分别为（77 668.34±27 682.79）和（64 585.83±26 173.62）（P<0.05），在有无转移组中IOD值分别为（63 854.73±21 730.42）和（77 218.05±28 930.01）（P<0.05），差异有统计学意义；并且肝癌及癌旁组织中FMNL-2 和MMP-2蛋白表达呈正相关（r=0.240，P=0.016）。结论 在肝癌发生、发展过程中存在FMNL-2和MMP-2蛋白异常表达，联合监测两者蛋白表达可能会有助于肝癌的诊断。
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关键词:
- 肝细胞癌 /
- 成蛋白相关蛋白2(FMNL-2) /
- 基质金属蛋白酶-2
Abstract: Objective To invesstigate the expression of FMNL-2 and MMP-2 in hepatocellular carcinoma (HCC) and the adjacent tissues, and to detect their relationship with the clinicopathological features. Methods The protein expression of FMNL-2 and MMP-2 were detected by immunohistochemistry MaxvisionTM method in the cancer tissues and adjacent tissues from 100 patients. Integrated optical density (IOD ) of immunohistochemical image was estimated by Image-ProPlus 6.0 software. Statistical analysis was performed by SPSS19.0 software. Results IOD of FMNL-2 between cancer tissues and adjacent tissues were (90 748.36±46 276.91)and (39075.46±24763.12);IOD of MMP-2 between cancer tissues and adjacent tissues were (87 969.46±27 131.77) and (59 517.72±20 218.23) (P<0.05); IOD of FMNL-2 between cirrhosis of liver tissue and non-cirrhosis liver tissue were(47 471.96±40 273.54) and (72 123.41±45 517.93), respectively(P<0.05); IOD of MMP-2 between HBsAg positive and negative group were (77 668.34±27 682.79) and (64 585.83±26173.62),respectively(P<0.05),IOD of MMP-2 between metastasis and without metastasis group were(63 854.73±21 730.42) and (77 218.05±28 930.01)(P<0.05),FMNL-2 expression was positively correlated to that of MMP-2 in cancer tissues and adjacent tissues (r=0.240,P=0.016). Conclusion FMNL-2 and MMP-2 proteins are abnormally expressed in hepatocellular carcinoma, indicating that detection of FMNL-2 and MMP-2 proteins may be helpful for diagnosis of hepatocellular carcinoma. -
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