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洛铂对骨肉瘤MG-63细胞增殖、凋亡的影响及其机制

冯和林, 郑丽华, 赵亚恒, 李增怀, 褚彦青, 冯建刚, 吴宏增, 单保恩

冯和林, 郑丽华, 赵亚恒, 李增怀, 褚彦青, 冯建刚, 吴宏增, 单保恩. 洛铂对骨肉瘤MG-63细胞增殖、凋亡的影响及其机制[J]. 肿瘤防治研究, 2014, 41(05): 358-362. DOI: 10.3971/j.issn.1000-8578.2014.05.002
引用本文: 冯和林, 郑丽华, 赵亚恒, 李增怀, 褚彦青, 冯建刚, 吴宏增, 单保恩. 洛铂对骨肉瘤MG-63细胞增殖、凋亡的影响及其机制[J]. 肿瘤防治研究, 2014, 41(05): 358-362. DOI: 10.3971/j.issn.1000-8578.2014.05.002
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FENG Helin, ZHENG Lihua, Zhao Yaheng, LI Zenghuai, CHU Yanqing, FENG Jian'gang, WU Hongzeng, SHAN Baoen. Effects of Lobaplatin on Proliferation and Apoptosis of Osteosarcoma MG-63 Cells and Its Mechanism[J]. Cancer Research on Prevention and Treatment, 2014, 41(05): 358-362. DOI: 10.3971/j.issn.1000-8578.2014.05.002
Citation: FENG Helin, ZHENG Lihua, Zhao Yaheng, LI Zenghuai, CHU Yanqing, FENG Jian'gang, WU Hongzeng, SHAN Baoen. Effects of Lobaplatin on Proliferation and Apoptosis of Osteosarcoma MG-63 Cells and Its Mechanism[J]. Cancer Research on Prevention and Treatment, 2014, 41(05): 358-362. DOI: 10.3971/j.issn.1000-8578.2014.05.002
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洛铂对骨肉瘤MG-63细胞增殖、凋亡的影响及其机制

  • 1. 050011石家庄，河北医科大学第四医院骨科；2. 河北医科大学研究生学院；3. 河北医科大学第四医院科研中心 河北省肿瘤研究所

基金项目: 河北省科技厅支撑课题资助项目（11276156）
详细信息
    作者简介:

    冯和林（1978-），男，硕士，主治医师，主要从事骨肿瘤研究与诊治工作

    通讯作者:

    单保恩，E-mail: baoenshan@hotmailom

  • 中图分类号: R738.1

Effects of Lobaplatin on Proliferation and Apoptosis of Osteosarcoma MG-63 Cells and Its Mechanism

  • 1.Department of Orthopedics,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;2.Graduate School of Hebei Medical University;3. Research Center, The Fourth Hospital of Hebei Medical University and Hebei Cancer Institute

摘要

    摘要
  • 摘要: 目的 探讨洛铂对骨肉瘤MG-63细胞增殖与凋亡的作用及其机制。方法 取对数生长期的MG-63细胞进行实验，分为对照组和实验组 (加入不同浓度洛铂: 2、4和8 μg/ml)。采用噻唑盐（MTT）比色法、流式细胞技术(FCM)检测洛铂对MG-63细胞的形态改变、生长及增殖抑制、细胞周期阻滞和细胞凋亡诱导等作用，并绘制不同浓度时MG-63细胞的生长曲线，应用Western blot的方法分析洛铂对MG-63细胞的Bcl-2蛋白表达的影响。结果 洛铂能够抑制MG-63细胞的生长、增殖并诱导其凋亡，并呈浓度和时间依赖效应。Western blot检测结果显示洛铂可使MG-63细胞Bcl-2蛋白表达下降。结论 洛铂能显著抑制MG-63细胞增殖，诱导细胞凋亡和细胞周期变化，可能与调节 MG-63细胞Bcl-2蛋白的表达有关。

     

    Abstract: Objective To study the effects of lobaplatin on the proliferation and apoptosis of osteosarcoma MG-63 cells and the mechanism of its function. Methods MG-63 cells in log phase were divided into control group and experimental groups. Experimental groups were treated with different concentrations of lobaplatin (2, 4 and 8μg/ml). MTT colorimetry and fl owcytometry were used to detect the effects of lobaplatin on cellular morphology change, cell growth, cell cycle, and the induction of apoptosis in MG-63 cells. Cell growth curves were studied at different concentrations of lobaplatin. Western blot was used to detect the effects of lobaplatin on the expression of Bcl-2 in MG-63 cells. Results Lobaplatin could inhibit the growth or proliferation and increase the apoptosis of osteosarcoma cell line MG-63 in a dose- and time-dependent manner. Bcl-2 protein level was decreased after lobaplatin treatment. Conclusion The effects of lobaplatin on the proliferation, the apoptosis and changing the cell cycle change of MG-63 cells might be related to its inhibition on Bcl-2 protein expression.

     

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出版历程
  • 收稿日期:  2013-02-20
  • 修回日期:  2013-06-19
  • 刊出日期:  2014-05-24

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