Abstract: Objective To observe the treatment action of gastrodin on chemotherapy-induced neuropathic pain in rats, and the changes of IL-1 β and TNFα expression in spinal cord, and to study the mechanism in the spinal cord level. Methods Vincristine was administered intravenously on alternate days to establish the model of chemotherapy-induced neuropathic pain, after the model was established successfully, gastrodin was administered intravenously, mechanical allodynia was measured using electronic Von Frey, heat hyperalgesia was measured by electronic heatingplaten instrument; The expression of IL-1β and TNFα was detected using ELISA technique. Results Vincristine-induced neuropathic pain model was established successfully on the eighth day, after one week treatment with different doses of gastrodin, mechanical pain and heat pain threshold were differently improved in different dose treatment groups compared with the model group (P<0.01, P<0.05); In model group, the expression of IL-1β and TNFα was enhanced signifi cantly in spinal cord(P<0.01). Conclusion Gastrodin could relieve the response to vincristine-induced pain. The mechanism of action may be related to inhibiting the expression of IL-1β and TNFα in spinal cord level.