Abstract: Objective To explore the clinical methods and effects of laparoscopy combined with gastroscopy resection on gastric stromal tumor patients. Methods Data of 48 patients with gastrointestinal stromal tumor treated by laparoscopy combined with gastroscopy resection from June 2011 to October 2012 were analyzed retrospectively. Results Forty-two patients with gastric stromal tumor received laparoscopically extragastric wedge resection by localization of gastroscope for 50-240 min, with an average of (92±45) min. The intranperative blood loss was 5-80 ml approximately, with an average of (30±18.6) ml. The mean postoperative hospital stay was 4-8 days and no postoperative complication was occurred. Among these 42 patients, the operations on 6 patients with tumors located near to the cardia (≤5cm) or pylorus (≤5cm) were successful without rupture of the cardia or pylorus. In 6 cases performed by gastroscopy, 4 received gastroscopic submucosal dissection, 2 received gastroscopic resection fi rstly and perforation in gastric wall due to the complicated location in muscular layer, clipping the gastric perforation with gastroscopy in one case and the laparoscopic suture repair was performed in the other. The diameter of tumor ranged from 1.0cm to 5.2cm, with an average of (3.2±1.8)cm. Pathologic analysis of 48 resected specimens showed 32 cases with extremely low risk, 12 cases with low risk, 3 cases with middle risk and 1 case with high risk. No case of recurrence or metastasis was found during a follow-up period for 2-18 months. Conclusion Laparoscopic combined with gastroscopic resection for gastric stromal tumor is a safe and effective therapy with many advantages, such as, short operation time, minimal invasive, fast recovery, good cosmetic results, and can be considered as the preferred option for treatment on patients with gastric stromal tumor with diameter < 5cm.