Effects of Gold Nano Particles on HIF-1α and VEGF mRNA Expression and Normalizing Vascellum in Hepatic Tumor of Nude Mice
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摘要: 目的 观察纳米金(goldnano particles,GNP)对裸鼠H22肝癌缺氧诱导因子-1α（hypoxia inducible factor, HIF-1α）、血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）的表达及肿瘤血管 的影响。方法 Balb/c裸鼠14只，肝癌造模后，随机分两组，每组7只实验动物。GNP组：从肿瘤内注入GNP（浓度为500 nmol/L）溶液0.2 ml；对照组：用等量0.9%氯化钠溶液处理，连续用药7d。超声多谱勒分析肿瘤血管形态，测量肿瘤血管直径和血液灌注量；处死裸鼠时测量肿瘤体积，原位杂交检测HIF-1α mRNA及VEGF mRNA表达。结果 (1)GNP组肿瘤体积(0.935±0.129)cm3较对照组(1.573±0.247)cm3下降（P<0.05）。(2)GNP组肿瘤血管直径（0.6397±0.1548）mm及血液灌注量（1.171±0.241）cm3较对照组血管直径（1.1000±0.3247）mm 和血液灌注量（2.357±0.408）cm3减少（P<0.05）。(3)GNP组裸鼠肝癌组织HIF-1α mRNA（15.3±7.4）%、VEGF mRNA（23.7±9.5)%，均较对照组[(67.2±13.1)%，(70.3±14.6)%]明显降低（P<0.05）。结论 GNP可以使裸鼠肝癌血管形态趋于正常，降低肿瘤血液灌注；抑制HIF-1α mRNA、VEGF mRNA表达，抗肿瘤生长。
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关键词:
- 纳米金 /
- 肝细胞癌 /
- 肿瘤血管 /
- 血管内皮细胞生成因子 /
- 缺氧诱导因子-1&alpha
Abstract: Objective To observe the effects of GNP (goldnano particles,GNP) on nude mice hepatic tumor vascular morphology and the expression of hypoxia inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) mRNA. Methods Hepatocellular cancer cell line H22 were injected subcutaneously into the right armpits of 14 Balb/c nude mice. When the diameter of transplanted tumor reached about 7-8 mm,they were randomly divided into 2 groups, and drugs with GNP or normal saline were injected respectively. For 7 days later, the tumor vascular diameter and blood volume were measured by Doppler ultrasound. When the mice were sacrificed, the tumor's size was calculated and the expression of HIF-1α and VEGF mRNA were detected by in situ hybridization. Results (1)The tumor's size in experiment group (0.935±0.129 cm3) was decreased in comparison with that in control group (1.573±0.247 cm3), there was significantly difference (P<0.05).(2) The tumor's vascular diameter and blood volume in experiment group were (0.6397±0.1548)mm and (1.171±0.241)cm3 respectively, they were all signifi cantly reduced in comparison with those in control group (1.1000±0.3247 mm, 2.357±0.408 cm3)(P<0.05). (3)The expression of HIF-1α mRNA (15.3±7.4%) and VEGF mRNA (23.7±9.5), were dramatically attenuated in comparison with those in control group [(67.2±13.1)%,(70.3±14.6)%] (P<0.05). Conclusion GNP can make nude mice tumor's vascular morphology better and decrease their blood volume, inhibit the expression of HIF-1α mRNA,VEGF mRNA, this situation is benefi t to restrain the tumor growth.-
Key words:
- Nanogold particles /
- Hepatic carcinoma /
- Tumor vascular /
- VEGF /
- HIF-1&alpha
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