The Study of P⁵³ Gene Mutation in Human Small Cell Lung Cancer

To discuss the molecuar mechanism of the small cell lung cancer (SCLC). P⁵³ gene mutation in SCLC of 14 paraffin embedded tissue section was observed by immunohistochemical staining (LSAB method) and polymerase chain reaction and single-strand conformation polymorsphism (pcr-SSCP) analysis. The results showed that the positive rate of immunohistochemical reaction was 64. 3% (9/14). of which 2 cases are, 7 cases are ～ 。 P⁵³ gene point mutation in exon 5, 6, 7 and 8 are respectively: 21. 4% (3/14) ；14. 3% (2/14)；14. 3% (2/14) ；7. 1 % (1/14). The total mutation rate is 57. 1 % (8/14). It was suggested that Comparatively high mutation rate of P⁵³ gene exists in SCLC,which might be the crucial gene of cause of the human lung carcinoma.