A Modified Method of Fluorescent Staining for the Apoptosis Detection fron Blood Tumor Cells

A very useful method,fluorescent（Hoechst33258）staining is improved to detect apoptosis in suspension cultured HL－60,,K562 cells in vitro.The procedure involving cell fixating,making slide,staining and detecting can be finished in 2h,and this allows a survey of several thousand cells by a quick microscopic scan even when the apoptosis rate is extremely low（ l%）.Consequently,this method should be an easy,fast,and highly reliable system for routine laboratory detecting of apoptosis in suspension cultured cancer cells.