THE EFFECT OF PROSTAGLANDIN E₁ AND SODIUM SELENITE ON THE DNA CONTENT IN A₅₄₉ CELLS IN VITRO

The effect of prostaglandin E₁ and Sodium Selenite on the DNA content in A₅₄₉ cells was investigated in vitro. The A_(549) cells were divided into four groups: Groupl was Cultured in the medium alone for control. Group Ⅱ, Ⅲ, Ⅳ were culturet in the medium addedwith 10ngPGE_1/ml, 10ngPGE_1 10ngPGE and 1μgSe/ml, 1μgSe/ml, respectively. By means of UNIVAR microscope photometer, it was found that DNA content in A_(549) cells treated with 10ngPGE_1/ml (group Ⅱ) or lμgSe/ml (groupⅣ) concentration was decreased(P0.01). By the determination of DNA content in each individual cell, we found that the location of cells in each phase compartment was changed. The peak in group Ⅳ moved to the left. The possible mechanisms of the inhibitory effect ty PGE_1 and Na_2SeO_ were discussed.