STUDY OF THE CORRESPONDING ANTIGEN OF A MONOCLONAL ANTIBODY (MGCI) AGAINST GASTRIC CANCER
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Graphical Abstract
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Abstract
The corresponding antigen of a monoclonal antibody (MGcl)against gastric cancer was purified and partally characterized. The MGcl was purified by passing throuth a DEAE cellulose column and covalently coupled to CNBr activated Sepharose 4B. By means of immunoaffinity chromatogrphy,the Corr- esponding antigen of MGcl was purified from gastric cancer tissue. The results of periodate oxidation,SDS-PAGEand Immunblotting studies suggest that the corresponding antigen of MGcl is a glycoprotein with molecular weight of 72 KD and 52 KD.
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