RESEARCH OF HPV16 DNA IN HUMAN INVASIVE CANCER OF UTERINE CERVIX

DNA extracted from the cervical tissues and cervical decidual, cell were detected by hybridization of nucleic acid with the probe of (α—32p)—dCTP labeled 7.9Kb HPV DNA. The positive rate of HPV16 DNA of decidual cell DNA was 65.2%, of cervical cancer tissues DNA was 66.7% and had no significant difference, but differences between cervical cancer and cervicitis, eithcr decidual cell DNA or tissue DNA hybridized with probe all had significant difference (former 65.2—66.7%, latter 26.1—28.5%), P0.01. The Physical state of HPV16 DNA in cervical cancer southern blot and twe-dimentional electrophoresis were evaluated and showed that HPV16 DNAwas integrated in cervical cancer cell genome.