A LONC-TERM OBSERVATION OF THE SHORT-TERM AFBI INDUCED HEPATOCARCINOGENESIS TEST MODEL AND THE EFFECTS OF GREEN TEA AND BHA ON THE LONG-TERM TEST

Two handred male wistar rats were divided into three exper imental groups (A～C) andpone control group (D). All groupsreceived the same basic program of the short-term AFBI-indueeb hepatoeareinogenesis test model, i. e., during the eriod of AFB_1 ip., group A, B and C were fed with0.5%BHA, 0.2% BHA ands% green tea diet respectively while the group D was fed with normal diet only. Rats in each group were sacrificed at the end of the short-term model (sth week) or at the end of the long-term odservation (92th week). Results showed not only r-GT foci were most numerous and largest but also the incidence of PLC was highest in control group, while these values were lower in green tea and BHA group. The results suggest: (1) This short-term test model is fairly suitable for investigating AFB_1-induced hepatocarcinogenesis. (2) The number and size of r-GT foci were in good corelation with the incidence of PLC, so they are reliable in this short-term model. (3) Both green tea and BHA have inhibitory effect on AFBl-induced hepatocarinogenesis.