A preliminary study of DNA ploidy and Ag-NOR counts on the prognostic evaluation of 67 patients with colonic cancer

Flow cytometry(FCM) and silver colloid staining technique have special valve for retrospective analyses of the tumors.The DNA ploidy and Ag-NOR counts in 67 paraffin-embeded samples were analyzed,5 or 7-year survival rate was 78.0%(32/41) and 39.0%(16/41) for the patients with DNA diploid tumor,while 26.9%(7/26) and 11.5%(3/26) for the patients with DNA non-diploid trmpr(p<0.O1 and p<0.05 respectively).The survival percentage at 5 or 7 yearswas 80.0%(28/35) and 40.0%(14/35) for the patients with Ag-NOR counts less thaw X±SD=6.9±1.O,while 34.4(11/32) and 15.6%(5/32) for those with Ag-NOR counts more than that(p<0.0l and p<0.05 respectively).DNA ploidy and Ag-NOR counts were closely related to prognostic factors of colonic cancer.DNA diploid was 97.7%(34/35) for the patients with Ag-NOR counts less than the mean of 6.9±1.O,while 21.9%(7/32) for the patients with Ag-NOR counts more than that(p<0.01).These findings suggest that DNA ploidy and Ag-NOR counts be considered as prognostic parameters of colonic cancer.