Preliminary Isolation，purification and Identification of osteosarcoma Associated Antigen

An osteosarcoma associated antigen (OSAA) was partially purified from human osteosarcoma tissue by means of hypertonic potassium chloride extract and immunoaffinity chromatogrphy. The OSAA was demonstrated as a glycoprotein with a molecular weight of 57 KD by the analyses of sodium dodecyl sulfate polyacylamide gel electrophoresis, westen blotting and periodic .acid-schiff reagent staining, and called it Ogp57. Following the antigen assay and comparing with the known related antigens, we suggest the Ogp57 may be a novel OSAA.