An quantitative evaluation of transforming growth factor-β_1 in tissues of prostatic cancer and BPH with sandwich ELISA

Abstract Prostate tissu extracts from 20 patients with prostatic carcinoma (PC) and 20patients with benign prostatic hyperplasia (BPH) were quantitatively assayed for expression of immunoreactive transforming growth factor-β1, (TGF-β1 ) with sand-wich ELISA. The content of TGF-β1, was significantly higher in PC (2416±790. 6ng/g tissue) than that in BPH (1934. 2±673.0ng/g tissue). While the content of TGF-β1 in 4 normal prostates (459. 3 ± 187. 18ng/g tissue) was much lower than that in both experimental groups. The findings suggest that TGF-β1 may be biologically important not only in the physiological process of prostate but also in the development of PC and BPH.