Establishment of multidrug resistance cell strain of liver cancer and reserch of its multidrug resistance mechanism

For serching establishing human liver cancer multidrug resistance cell strain and reserch its drug resistance mechanism,the BEL-7402 cells were cultured orubicin by progressively cell strain BEL-7402/Dox increase concentration method,and the drug with Dox-resistance were selected for a resistance increased of BEL-7402 cells to vincristinelong period screening cultural.The (VCR) and other 8 anticancer drugs 27-1100 times respectivly by MTT assay.The MDR1 expression product membrane efflux p-glycoprotein and multidrug resistanceassociated(MRP)as well as GSH/GST were examined,the MDR and MRP gene expression were also detected using RT-PCR.The results showed that p-gp level of BEL-7402/Dox were 93.5 to 97.4%MRP were 84.7 to 90.2%,the level of MDR and MRP mRNA expression were also relatively high,no over experssion of GSH/GST was found.