Synergistic Activity of TNF-a with Superoxide Anion，SOD or Hydroxyl Radical on Human bladder Cancer

To investigate synergistic effects of tumour necrosis factor-a(TNF-a) with superoxide anion，SOD or hydroxyl radical on human bladder cancer. Human bladder cancer cell line BIU一87 was cultured.The synergistic action of superoxide anion，SOD or hydroxyl radical in combination was tested by MTT assay.The growth of BIU一87 cell was inhibited by TNFa in dose dependent manner.The cytotoxocity of TNF-a to BIU87 cell was enhanced by superoxide anion and inhibited by SOD.However，the combination of TNF-a and hydroxyl radical did not showed this sygnergistic action.This study demostrated that superoxide anion-TNF-a showed synergistic action against the BIU-87 cell line.