Studies of deletion of Rb gene in HCC

LOH of Rb gene at intron 1 was detected by PCR-RFLP in 24 HCCs·25%<3/12) LOH was found.At same time we also analyzed the structural alteration of the Rb gene from 10 cases by Southern hybridization using the Rb cDNA. The deletion was found at 4.5kb band in one case，at 9.8kb and 7.5kb bands in another case.All the cases with Rb gene abberation were found only in markedly advanced HCC.It suggest that deletion of Rb gene may participate in the progression of HCC.