The effects of GM-CSF on the proliferation and invitro cytotoxicity of TILs with gastric carcinoma

The effects of GM-CSF on the proliferation and cytotoxicity of TILs with gas-tric carcinoma was investigated .The results demonstrated that GM-CSF alone did not stimulate TIL proliferation.but with IL (400u/ml)，GM-CSF promoted TIL proliferation，expansion.The cytolytic activity (MTT method)showed GM-CSF enhanced TIL in vitro cytotoxicity to autologous tumor cells or allogenic tumor cells.The possible mechanisms related to the effects of GM-CSF on TILs were dis-cussed.