Establishment and Application of Transplantable Model of Human Esophageal Squamous Cell Careinoma in Nude Mice

A model of the transplantation of human esophageal careinoma in nude mice (Eca9l0709) was established by the direct transplantation of a surgical specimen from a patient with esophageal squamous cell careinom a subeutaneously in the NIH nude mice. Light and electron microseopy showed that the morphology was similar to that of the original tumor. Alu sequence was present in the transplanted tumor, indicating that transplanted tumor was of human origin. Bleomycin was A₅ used for the treatment of Eca910709. Eeperimental results showed that Bleomycin A₅ exhibited a remarkable growth inhibition on the Eca910709. The inhibition rates of separate experiments were 58% and 70%(P<0.01). The Eca910709 exhibited resistance to Bleomycin A₅: afrer three courses of treatments. The inhibition rates were significantly decreased from 70%(P<0.01) to 34%(P0.05). Inereasing the dose of Bleomycin A₅. It has exhibited that Bleomycin A₅ can inhibit tumor in resistant the Eca910709 to a certain drgree. The inhibition rate was 44%(P<0.01).