Study on the role of P53 gene transfer on human glioma cell growth in nude mice
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Graphical Abstract
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Abstract
Objective To explore the significance and role of P53gene transfer on human glioma cell growth in nude mice. Method Wild-type P53 gene(wt-P53)and mutant P53 gene(mt-P53)were transfected into the human glioma cell line SHG44.And the growth of gene-transfected cell lines were observed in vitro and in vivo. Results The tumor resulting from the cells transfected with the wt-P53 gene more slowly and was smaller than that from control SHG44 cells.In contrast,the tumor from the ce-lls transfected with the mt-P53 gene grew faster than that produced by cells transfected with the Wt-P53 gene and that produced by control SHG44 cells. Conclusion The wild-type P53 gene could inhibit the glioma cell growth in nude mice and the mutant P53 gene could enhance the glioma cell growth in nude face.
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