Establishment of an model of human gastic cancer in nude mice via orthotopic transplantation

Objective: to establish an orthotopic transplant model of human stomach cancer and compare two different approaches. Methap: Eighteen BAL/C-nu/nu nude nudce were randomly devided into three groups: for the first group, SGC-7901 human gastric tu-mor tissue pieces were transpianted into a small "pouch" in stomach wall; for the secondgroup,tumor tissue pieces were fixed in the stomach wall directly; and the third groupwas the control. All the tunlor - bearing mice underwent autopsy, Results: Both ap-proaches yielded 100% tunror take rate, 100 % local invasiveness however, liver metasta-sis were higher in the first group than the second one (66.7% vs 33.3%, P<0.00l )and so were pylorochesis and ascites(83.33%　vs 16.6%, P<0.00l; 66.7% vs 16.6%, P<0.001, reSPectively). Conclusion: the medel grew more "patient-like" when tunortissue pieces were implanted into the small artificial pouch.