Relationship of human papillomavirus types 16 and 18 infection and p53 gene mutation in lung squamous cell carcinoma

Objective To study the relationship of human papillomavirus (HPV) and p53 gene mutation in the pathogenesis of lung squamous cell carcinoma. Methods PCR-SSCP-EB method was used to detected the mutation of exons 5 to 8 of p53 gene,and the HPV types 16,18 DNA related sequences were detected by PCR. Results 67.5% of 40 tumor tissue specimens had p53 gene mutations. The incidences of mutation of exons 5 to 8 were 17.5%,12.5%,17.5% and 20%,respectively. 42.5% of lung squamous cell carcinoma samples were shown...