Study of VEGF Antisense Oligodeoxynudeotide and Daunorubicin to Increase the Sensitivity of AML and CML Cells

Objective To explore the effect of VEGF antisense on sensitivity of AML and CML cells to Daunorubicin (DNR). Methods A7, which was the most effective one of AS PS ODN selected with help design of computer and experimental assay, contains 20 mer modified with phosprothioate and was transferred into cells mediated with lipofectin. 24 hours later, DNA was added into culture system for continuing incubation for 72h. And then the viable cells were detected with trypan blue exclusion every 24 hours, cell ap...