Comparison of Serum-Free Medium AIMV with Standard Serum-Containing Medium in Proliferation and Excretion Cytokine Level of CIK Cells in Vitro

Objective To compare serum-free medium AIMV with standard serum-containing medium in proliferation CIK cells and excretion cytokine level in vitro. Methods CIK cells were generated by culture CBMNCs in the presence of four cytokines(IFN-γ,IL-2,IL-1 and OKT3) in serum-free medium AIMV or in standard serum-containing medium separately. Proliferation, phenotypes, excretion cytokine level were compared. Results Compared with cells cultured in standard serum-containing medium, cell cultured in serum-free medium AI...