An experimental study of human membrane-bound hyaluronidase (PH-20) on breast cancer cell (MDA231) growth and metastasis

Objective To study the effects of human sperm membrane-bound hyaluronidase (PH-20) on gene transfected MDA231 cells growth and metastasis. Methods PH-20 cDNA was transfected into human breast cancer cell line MDA231.Transfectants (MDA231-PH-20) were then implanted onto chorioallantoic membrane (CAM). Meanwhile, empty vector pcDNA3 was transfected into MDA231 (MDA231-pcDNA3) as control. The followings were performed for the tumor growth and metastasis study: (1) weighting and measuring the tumors; (2)...