Human telomerase reverse transcriptase (htert) activity correlates with c-myc expression and regulation in hepatoma cell lines

Objective To investigate the activity of human telomerase reverse transcriptase (htert) and the expression of c-myc in hepatoma cell lines, which may regulate the pathway in hepatic tumourigenesis. Methods TRAP-ELISA method was used to determine the telomerase activity in hepatoma cell lines, and the expression of htert and c-myc in transcriptional level was measured by using RT-PCR technique. Then the c-myc expression plasmid and the vector which harboring htert promoter were co-transfected into C...