Identification of Functional Antibodies of Lung Cancer by Screening of Functional Antibody Library

Objective 　Monoclonal antibodies which inhibit lung cancer may be candidate drugs for the antimalignancy therapy. Methods 　Malignant cells were separated form lung carcinoma tissues to immunize Balb/c mouse. The spleen cells of the immunized mouse were fused with SP2/0 cells and culture on the methyl cellulose. Antibodies of each clone were screened by immunofluorescence, immunohistochemistry, ELISA, cell proliferation assay, tumor cell invasion assay and adhesion assay. Functional antibodies were also studied by tumor treatment experiments in vivo. Results 　One thousand five hundred and seventy-three monoclonal antibody clones were obtained by the fuse of spleen cells with SP2/0 cells. Forty-one clones could significantly suppress the lung cancer cell proliferation, 24 monoclonal antibody could inhibit tumor cell invasion in Mat rigel, and 15 monoclonal antibody could decrease the adherence between lung cancer cells and Collagen I. I n vivo assay further demonst rated that two of these functional antibodies could significantly inhibit lung tumor growth in mice. Conclusion 　By using functional antibody library, we successfully obtained functional monoclonal antibodies which suppressed the lung cancer cells in vitro and in vivo. These antibodies may be applied in anti-tumor therapy of lung carcinoma.