Expression and Its Signif icance of Lipoxygenase in Gastric Carcinoma

Objective 　To test the expression of 5-lipoxygenase and 15-lipoxygenase-1 (15-LOX-1) mRNA and protein in gast ric carcinoma and adjacent normal tissue, and to investigate their relationship. Methods The 5-LOX and 15-LOX-1 mRNA and protein level were detected in pairs of gastric carcinoma and adjacent normal tissue by reverse transcription polymerase chain reaction (RT-PCR) and western blot methods. Results 　In gastric carcinoma, the expression of 5-LOX mRNA and protein was significantly higher than that in the adjacent normal tissue ( P < 0. 05), while the expression of 15-LOX-1 in gastric carcinoma was significantly lower than that in the adjacent normal tissue ( P < 0. 05) . No significant correlation was found between 5-LOX and 15-LOX-1 ( P > 0. 05) . Conclusion 　In gastric carcinoma, the expression of 5-LOX and 15-LOX-1 lost their balance, which was possibly correlated to the genesis and progress of gastric carcinoma. There was with close correlation between them.