Cytokines Production of Murine Spleen Cells Induced by Gene Transfer of 4-1BBL into Hepatocellular Carcinoma Hepa1-6 in Vitro

Objective 　To study the cytokines production of spleen cells induced in vitro by murine 4-1BBL gene transfected Hepa1-6. Methods 　Murine hepatocellular carcinoma cell line Hepa1-6-m4-1BBL which could highly express murine 4-1BBL was cultivated previously. The tumor cell vaccine ( TCV-m4-1BBL) was obtained by treating Hepa1-6-m4-1BBL with mitomycin (MMC) . Cocultivation TCV with syngeneic murine spleen cells and the supernatant s were harvested for detecting the cytokines ( IL-2, TNF-α and GM-CSF) . Results 　Comparing with TCV-Hepa1-6, high levels of IL-2, TNF-α and GM-CSF were released by the spleen cells after stimulated by TCV-m4-1BBL. Conclusion 　Stimulation signals delivered by 4-BBL remarkably increased the production of IL-2, TNF-α and GM-CSF.