Effect of Zedoary Oil for Apoptosis and Radiotherapy in Human Nasopharyngeal Carcino2 ma Cell Line CNE22

Objective 　To explore the Zedoary oil, Zedoary oil jointγ2ray irradiation on human nasopharyn2 geal carcinoma (NPC) cell lines CNE22 inhibiting the proliferation and induction of apoptosis ; analysis of whether the Zedoary oil enhancement of the role of radiotherapy. Methods 　MTT colorimet ric assay and elect ronic microscope method were employed to examine the growth status and apoptosis of CNE22 cells. Flow cytomet ry was employed to detect the effect of Zedoary oil 、Zedoary oil and/ or γ2radiation for growth inhibition in CNE22 cells. Results 　Zedoary oil used alone, the CNE22 cell proliferation is the exact effect, and all with conventional chemotherapy drug 52Fu a similar effect ( P > 0. 05) ;Zedoary oil drugs inhibit a concent ration2dependent, the minimum effect f rom the concent ration of 10μg/ ml, the best time of 48 hours ; flow cytomet ry showed that 10μg/ ml, 100μg/ ml, 300μg/ ml Zedoary oil2t reated CNE22 cells the apoptosis ratio of were 0. 5 %, 2. 15 %, 10. 2 % on 48 hours, respectively. In the group of t reatment with 10μg/ ml, 100μg/ ml, 300μg/ ml curcuma and 100cGyγ2radiation, the apoptosis ratio were 8. 6 %, 14 %, 26 % on 48 hours, respectively. And the average mortality rate of cells were below 5. 0 %. They has the synergistic action for apoptosis in CNE22 cells ( P < 0. 01) . Combined 96 h af ter the test group of cells to death based, less apoptosis ;cell death was induced by 500μg/ ml curcuma. Zedoary oil is the CNE22 cell block in G1 phase. Elect ron microscopy apoptosis ult rast ructure can see that zedoary oil can be induced by CNE22 cells apoptotic body, leading to apoptosis. Conclusion 　Zedoary oil to increase the in vit ro method role in CNE22 NPC cells can inhibit cell proliferation and induced apoptosis ; Zedoary oil and small doses ofγ2ray joint role obviously synergy ; mechanism and it s role in apoptosis induced by tumor cells and af2 fect cell growth cycle.