Apoptosis in HeLa Cells Induced by Antisense Oligodeoxynucleotide of Human survivin Gene

Objective 　To investigate the apoptosis in HeLa cell induced by antisense oligodeoxynucleotide of human survivin gene, and the relation between this apoptosis and expression of Bcl22 and Bax. Methods 　In vi t ro experiment s, TUNEL staining method was used to detect the apoptosis status of cervical canc2 er HeLa cell line t reated by antisense oligodeoxynucleotide of survivin. Immunohistochemical staining was used to detect the expression of apoptosis2relation gene Bcl22 and Bax. Results 　Antisense oligodeoxy nu2 cleotide of survivin was able to induce the apoptosis in HeLa cells. Antisense oligodeoxynucleotide of sur2 vivin can reduce the expression of apoptosis2relation gene Bcl22 and increase the expression of apoptosis2 relation gene Bax. Conclusion 　Antisense oligodeoxynucleotide of survivin was able to induce the apopto2 sis in HeLa cell . This apoptosis may be mediated by down2regulation of apoptosis2regulated gene Bcl 22 and up 2regulation of Bax.