Study on the Relationship between the Behavioral Abnormality of Chromosomal Telomeres inMalignant Neoplasm 's and the Carcinogenesis

Objective Exploration of the relationship between the behavioral abnormality of chromosomal telomeres in malignant neoplasm's and the carcinogenesis . Methods The telomeric association was used by peripheral blood lymphocyte cultures, the telomere length was used by means of southern hybridization, the telomerase activity was used by means of TRAP ( telomeric Repeat Amplification protocol )on PCR. Results The telomeric association rate of esophageal carcinoma (14.38% ), Nasopharyngeal Carcinoma NPC (35.07% ), Lung Carcinoma (24.40% ) were found to be higher than that of controls (9.83% 、20.27% and 14.29% ) P <0.01, the telomere length of in NPC and lung carcinoma 16.67% and 16.67% have telomere elongation, 76.67% and 69.04% have telomere reduction, 6.67% and 14.29% exhibited equal lengths as compared with the controls . The positive rates of telomerase activity were 82.14% in NPC, 78.3% in NSCLC 、100% in SCLC. Condusion Increase of the telomeric association retes, the reduction of telomere length and the positive rates of telomerase activity were more closely related to cell carcinogenesis .