Cloning and Sequencing of Human Papillomavirus Type 18 L1 Gene from Cervical Cancer Tissue

Objective To clone and analyze the whole sequence of L1 gene and its deduced L1 protein of HPV18 from specimen of cervical cancer. Methods HPV18 L1 gene was amplified from a HPV18-positive DNA sample of a Chinese patient with cervical adenocarcinoma by means of PCR and then cloned and sequenced. Results In comparison with the prototype, there were twelve point mutations in HPV 18 L1 nucleic acid sequence and the differed sequence had changed the triplet codes. Therefore, the amino acid sequence of HPV 18 L1...