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ZHANG Zhi-yun, ZHENG Qi-chang, DAI Zong-qing, ZHANG You-shun, ZOU Can, HUANG-Ling, WANG-Ju. Construction and Application of Recombinant Adenovirus Vector Expressing the MDR1 shRNA[J]. Cancer Research on Prevention and Treatment, 2006, 33(11): 798-801. DOI: 10.3971/j.issn.1000-8578.377
Citation: ZHANG Zhi-yun, ZHENG Qi-chang, DAI Zong-qing, ZHANG You-shun, ZOU Can, HUANG-Ling, WANG-Ju. Construction and Application of Recombinant Adenovirus Vector Expressing the MDR1 shRNA[J]. Cancer Research on Prevention and Treatment, 2006, 33(11): 798-801. DOI: 10.3971/j.issn.1000-8578.377
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Construction and Application of Recombinant Adenovirus Vector Expressing the MDR1 shRNA

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  • Objective To construct a recombinant adenovirus vector expressing the MDR1 shRNA and investigate its effect in human hepatocellular carcinoma cell. Methods According to the MDR1 mRNA sequence, the DNA segment homogenizing wish MDR1 shRNA was synthesized and cloned into the shuttle plasmid pshuttle-MDR1. The later and adenovirus vecter were cotransfected to package the pAd-MDR1. pAd-MDR1 was transfected into human hepatocellular carcinoma cell SMMC-7721. The expression of P-gp on the cellular membrane wasdet...
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