Molecular Mechanisms Involved in Regulation of Proliferation and Apoptosis by Stat5 Antisense Oligonucleotide and AG490 in Colon Cancer Cells

Objective In this study, we show that inhibition of the Stat5 signaling pathway using Stat5 AS-ON and AG490 significantly suppress the growth of colon cancer cell lines harboring constitutive active Stat5. Methods Stat5 AS-ON, AG490, or Stat5 AS-ON+AG490 was added into culture media, followed by MTT assay, the absorption value was measured and the growth curve was drawn; western blot analysis was performed on colon cancer cell lines; flow cytometry was applied to analyze the cell cycle and apoptosis. Result...