Antisense Oligodeoxyribonucleotide Target Ki-67 Inhibits Proliferation of Human Hepatocellucar Carcinoma Cell HEPG-7402 in Vitro

Objective To investigate phosphorothioated antisense oligodeoxynucleotide (ASODN) target Ki-67 decreasing the antigene s expression and inhibiting proliferation of HEPG-7402 cell in vitro. It will show us a new method to cure hepatocellular carcinoma by genetherapy in the future. Methods We treat HEPG-7402 cells with ASODN and observe changes of cell s proliferation by the way of MTT.ICC technique was used to find the change of cell s Ki-67 labeling index (LI). RT-PCR method was used for detecting Ki-67 mRN...