Antitumor Efficacy of Human Endostatin Expressed by Replication-Deficient Adenovirus on Human Breast Cancer in Nude Mice

Objective To establish human breast cancer xenografts in nude mice, and to investigate the antitumor efficacy of human endostatin expressed by replication-deficient adenovirus Ad-hE in vivo. Methods The mammary cancer cells MCF-7 were injected subcutaneously in BALB/c nude mice to establish the xenografts. The tumor growth was observed and measured after Ad-hE treatment. The immunohistochemistry was used to examine the expression of endostatin in cancer cells and to calculate the density of microvessels in ...