PEI Delivered PKC-α ASODN Enhancing Chemotherapeutic Sensitivities on SMMC-7721 Cell Line

Objective 　To investigate if the PKC2α ASODN delivered by polyethyleneimine ( PEI) can en2 hance the drug2sensitivities of 52fuorouracil (52Fu), arsenic t rioxide (As2O3 ) and ( HCPT) on human hep2 atocellular carcinoma SMMC27721 Cell line. Methods 　SMMC27721 cell incubated with different concen2 t ration of 52Fu, As2O3 and HCPT alone or combing with ASODN or PEI2ASODN, the inhibitory rate of cell proliferation was tested by using a cell counting kit (WST28 method) and IC50 was calculated. Results Three chemotherapeutic drugs combined with PEI2ASODN (0. 25μmol/ L) reduced their IC50 to 3. 9μg/ml 、2. 67μmol/ L 、1. 46μg/ ml respectively, and their chemotherapeutic sensitivities was enhanced 2. 86, 1. 84 and 4. 01 times compared with the pure chemotherapeutic drug group. Conclusion 　PEI2ASODN can enhance the chemotherapeutic sensitivity on SMMC27721 cell line, and HCPT get s the best combined effect of all.