Inhibition of Proliferation and Induction of Apoptosis by Freeze Combined with rhTNF-α in Rat C6 Brain Glioma Cell

Objective To investigate the effect of freeze combined with rhTNF-α on the cell apoptosis and growth of rat C6 brain glioma. Methods At 15th day after rat C6 brain glioma model being established,experiment animals were divided randomly into 4 groups: (G1)saline group; (G2)cryotherapy group; (G3) rhTNF-αgroup; (G4)combined group.And the corresponding treatment was performed according to the different groups.At 15th day following treatment,TUNEL and FCM were applied to detect apoptosis.PCNA expression was exam...