Alternating Splicing of Tumor Suppressor Gene CEACAM1 is Correlated with Over-expression of PTB in Lung Cancer

Objective The possibility of modulating the difference of CEACAM1 splicing pattern between tumor tissues and their corresponding non-malignant lung tissue. Methods Detect the expression pattern of CEACAM1 in non-small cell lung cancer (NSCLC) by Hot-PCR,mini-gene constructs and co-transfection with PTBs. Results The RNA transcript from this mini-gene could be spliced in a cell-specific manner.The exon 7 was predominantly included in H1944,but predominantly excluded in 22B.Over-expression of PTB could ubiquito...